Luk et al. [20] |
2012 |
Somatosensory cortex and dorsal neostriatum |
30 and 90 days post injection (p.i) |
M83 mice maintained on a C57BL/C3H background, α-syn -/- mice maintained on a C57BL/6 background |
5 μg of 5 mg/mL human α-syn1-120Myc or WT full-length human α-syn per brain |
Luk et al. [18] |
2012 |
Striatum |
30, 90, and 180 days p.i |
C57BL6/C3H F1, C57BL6/SJL F1, and CD1 mice |
5 µg of 5 mg/mL full-length WT mouse α-syn per hemisphere |
Masuda-Suzukake et al. [40] |
2013 |
Substantia nigra |
15 months p.i |
4- to 6-month-old female C57BL/6J mice |
10 µg of mouse or human α-syn PFFs |
Guo et al. [60] |
2013 |
Hippocampus |
3, 6, and 9 months p.i |
2- to 3-month-old PS19 mice or C57BL6/C3H F1 mice |
5 μg of PFFs |
Sacino et al. [49] |
2014 |
Hippocampus |
1, 2, and 4 months p.i |
2-month-old M83 mice and M20 mice |
2 μL of 2 mg/mL hfib 21–140 or Δ71–82 α-syn |
Sacino et al. [27] |
2014 |
Intramuscular (biceps femoris) |
4, 8, and 12 months p.i |
2-month-old M83 mice and M20 mice |
10 μg of mouse PFFs, human 21–140 α-syn PFFs or Δ71–82 α-syn |
Paumier et al. [48] |
2015 |
Striatum |
30, 60, and 180 days p.i |
Adult male Sprague-Dawley rats |
8 μg of mouse or human α-syn PFFs |
Osterberg et al. [39] |
2015 |
Cortex |
2, 3, and 4 months p.i |
2- to 3-month-old α-syn-GFP mice |
2.5 µL of 2 mg/mL mouse WT α-syn PFFs |
Peelaerts et al. [57] |
2015 |
Substantia nigra |
4 months p.i |
Female Wistar rats |
2 µL of 5 µg/µL α-syn PFFs (total of 10 µg) |
Breid et al. [30] |
2016 |
Peritoneum, tongue |
Up to 420 days p.i |
Tg (M83+/−:Gfap-luc+/−) and (Gfap-luc+/−) mice |
5 μL (intraglossal) or 50 μL (intramuscular) of sonicated α-syn PFFs |
Thakur et al. [45] |
2017 |
Substantia nigra, ventral tegmental area |
10 days p.i |
Female Sprague-Dawley rats |
2.5 μL of full-length recombinant human α-syn PFFs (10 μg total) per site |
Karampetsou, et al. [21] |
2017 |
Striatum |
60 days p.i |
2- to 4-month-old male and female wild-type C57BL6/C3H mice and α-Syn-/- mice (C57BL6/JOlaHsd mice) |
4.25 μg (4 μL) of three different types of human recombinant fibrillar α-syn |
Abdelmotilib et al. [35] |
2017 |
Striatum |
6 months p.i |
8- to 10-week-old mice and rats |
10 μg (mice) or 20 μg (rats) of mouse or human α-syn PFFs |
Blumenstock et al. [43] |
2017 |
Dorsal striatum |
30, 60, and 90 days p.i; 5 and 9 months p.i |
2-month-old mice |
5 μL (25 μg) of mouse α-syn PFFs |
Sorrentino et al. [56] |
2017 |
IC or CPu |
4–5 months p.i |
4-month-old M20 non-Tg (NTG) mice |
2 μL of 2 mg/mL human or mouse WT α-syn PFFs |
Harms et al. [63] |
2017 |
Substantia nigra |
1, 3, and 6 months p.i |
8- to 10-week old Sprague-Dawley rats |
8 μg of mouse α-syn PFFs |
Shimozawa et al. [50] |
2017 |
Caudate and putamen |
3 months p.i |
26-month-old marmosets |
50 μL aliquots of 4 mg/mL mouse α-syn PFFs |
Okuzumi et al. [44] |
2018 |
Striatum |
1 week pi, 0.75, 1.5, 3, and 6 months p.i |
2- to 3-month-old C57BL/6J mice |
5 μg/2.5 μL of recombinant mouse or human α-syn PFFs |
Rey et al. [29] |
2018 |
Olfactory bulb |
1, 2, 3, 6, 9, 12, 18, and 23 months p.i |
3-month-old female C57BL/6J mice |
8 μL of 1 mg/mL WT mouse or human α-syn PFFs |
Uemura et al. [28] |
2018 |
Mouse gastric wall |
45 days p.i |
2-month-old male C57BL/6J mice |
3 μL of 2 μg/μL mouse α-syn PFFs |
Duffy et al. [47] |
2018 |
Striatum |
6 months p.i |
2-month-old male Fischer mice |
2 μL of 2 μg/μL mouse α-syn PFFs (unilateral) |
Sorrentino et al. [42] |
2018 |
Muscle |
1, 2, 3, and 4 months p.i |
2-month-old M83+/- mice |
2,5, or 10 μg of mouse α-syn fibrils |
Milanese et al. [41] |
2018 |
Striatum |
4 months p.i |
C57BL/6 mice |
5 μg (2.5 μL) of human α-syn PFFs |
Terada et al. [46] |
2018 |
Striatum |
3 months p.i |
10-week-old C57BL/6J mice |
5 μL (150 μM) of human WT or truncated α-syn PFFs |
Ayers et al. [54] |
2018 |
Sciatic nerve (unilateral) |
1, 2, and 4 months p.i and end-stage |
M83 and M20 mice |
2 μL of 2 mg/mL mouse WT or human Δ71–82 α-syn PFFs |
Manfredsson et al. [55] |
2018 |
Enteric neurons of the descending colon |
1, 6, and 12 months p.i |
Young adult male Sprague-Dawley rats |
5 μg of 2 μg/μL mouse or human α-syn PFFs |
Manfredsson et al. [55] |
2018 |
Colon and stomach |
12 months p.i |
Non-human primates (Macaca fascicularis) |
10 injections of 10 µL (2 µg/µL) |
Peralta Ramos et al. [68] |
2019 |
Vein |
12 hours p.i |
2-month-old C57BL/6 mice |
5 μg of Atto 488-labeled α-syn fibrils or ribbons |
Durante et al. [77] |
2019 |
Striatum |
6, 7 weeks p.i |
Male Wistar rats |
1 µL of human α-syn PFFs per site |